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This practical, extensively illustrated handbook covers the procedures that are undertaken in andrology and ART laboratories to analyse and assess male-factor infertility, and to prepare spermatozoa for use in assisted conception therapy. The content is presented as brief, authoritative overviews of the relevant biological background for each area, plus detailed, step-by-step descriptions of the relevant analytical procedures. Each technical section includes quality control considerations and the optimum presentation of results. In addition to the comprehensive 'basic' semen analysis, incorporating careful analysis of sperm morphology, the handbook provides established techniques for the use of computer-aided sperm analysis and sperm functional assessment. The interpretation of laboratory results in the clinical context is highlighted throughout, and safe laboratory practice is emphasized. Fully revised, incorporating the new ISO TS 23162 on basic human semen analysis throughout, this is an invaluable resource to all scientists and technicians who perform diagnostic testing for male-factor infertility.

Semen --- Andrology --- Examination --- Laboratory manuals. --- Generative organs, Male --- Human reproduction --- Men --- Body fluids --- Exocrine glands --- Spermatozoa --- Testis --- Endocrine aspects --- Diseases --- Physiology --- Secretions --- Semen Analysis --- Infertility, Male --- Spermatozoon --- X-Bearing Sperm --- X-Chromosome-Bearing Sperm --- Y-Bearing Sperm --- Y-Chromosome-Bearing Sperm --- Sperm --- Sperm, X-Bearing --- Sperm, X-Chromosome-Bearing --- Sperm, Y-Bearing --- Sperm, Y-Chromosome-Bearing --- Sperms, X-Bearing --- Sperms, X-Chromosome-Bearing --- Sperms, Y-Bearing --- Sperms, Y-Chromosome-Bearing --- X Bearing Sperm --- X Chromosome Bearing Sperm --- X-Bearing Sperms --- X-Chromosome-Bearing Sperms --- Y Bearing Sperm --- Y Chromosome Bearing Sperm --- Y-Bearing Sperms --- Y-Chromosome-Bearing Sperms --- Sub-Fertility, Male --- Subfertility, Male --- Sterility, Male --- Male Infertility --- Male Sterility --- Male Sub-Fertility --- Male Subfertility --- Sub Fertility, Male --- Sperm Injections, Intracytoplasmic --- Semen Quality Analysis --- Semen Quality --- Analyses, Semen Quality --- Analysis, Semen Quality --- Qualities, Semen --- Quality Analyses, Semen --- Quality, Semen --- Semen Analyses --- Semen Qualities --- Semen Quality Analyses

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Azoospermia, defined as the absence of sperm in the ejaculate after examination of the centrifuged specimens, affects about 1% of the male population and 10–15% of infertile men. In about two-thirds of cases, this is caused by severe spermatogenic dysfunction, and it is commonly termed “nonobstructive azoospermia” (NOA) to differentiate it from the less severe form of azoospermia caused by the obstruction of the seminal tract (obstructive azoospermia—OA), the latter affecting the remaining one-third of cases. Managing patients with NOA is challenging due to the severity of spermatogenic dysfunction and the lack of medical treatments, with surgical retrieval of testicular sperm being the only way of enabling some of these patients to father their own biological children. In-depth clinical knowledge is key for supporting clinical reasoning and decision making when counselling patients with NOA, and surgical skill is required to maximize the outcome of surgical procedures that aim to retrieve testicular sperm. The present book is a collection of scientific papers published in a Special Issue of the Journal of Clinical Medicine, authored by some of the most influential researchers in the field. The Special Issue, and thus also this book, were conceived to provide early career reproductive urologists and endocrinologists with an update of the scientific evidence in the field, together with surgical tips.

Medicine --- Pharmacology --- nonobstructive azoospermia --- micro-TESE --- FSH treatment --- hormonal treatment --- testosterone level --- microdissection testicular sperm extraction --- non-obstructive azoospermia --- management --- infertility --- intracytoplasmic sperm injection --- testicular azoospermia --- sperm selection --- sperm --- cryopreservation --- in vitro maturation --- azoospermia --- diagnosis --- male infertility --- spermatogenic failure --- testis biopsy --- sperm retrieval --- genetic testing --- endocrine evaluation --- review --- hypogonadism --- Sertoli cell-only syndrome --- testicular spermatozoa --- processing --- microfluidics --- new technologies --- genetics --- exome --- WES --- Y chromosome --- cancer --- NOA --- genes --- general health --- ICSI --- offspring health --- microTESE --- prediction model --- n/a

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This book presents the latest advances in assisted reproductive technologies applied to ruminants, such as multiovulation, artificial insemination, embryo transfer, and in vitro fertilization (IVF), which have been useful tools to accelerate the genetic progress in these species. The reader will find detailed studies on bisons, bovines, and goats

sperm viability --- embryo production --- fertilization --- blastocyst --- caprine --- COC --- IVF --- IVP --- IVEP --- oocyte competence --- photoperiod --- reproductive efficiency --- season --- seasonal breeder --- HSP70 --- heat stress --- in vitro embryos --- gene expression --- cattle --- Holstein --- Mediterranean Water Buffalo --- in vitro embryo production --- laparoscopic ovum pickup --- accelerated genetic gain --- prepubertal --- embryo development --- wisent --- European bison --- oocytes --- sperm --- embryos --- embryo transfer --- germplasm bank --- threatened species

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This book presents the latest advances in assisted reproductive technologies applied to ruminants, such as multiovulation, artificial insemination, embryo transfer, and in vitro fertilization (IVF), which have been useful tools to accelerate the genetic progress in these species. The reader will find detailed studies on bisons, bovines, and goats

Research & information: general --- Biology, life sciences --- Animals & society --- sperm viability --- embryo production --- fertilization --- blastocyst --- caprine --- COC --- IVF --- IVP --- IVEP --- oocyte competence --- photoperiod --- reproductive efficiency --- season --- seasonal breeder --- HSP70 --- heat stress --- in vitro embryos --- gene expression --- cattle --- Holstein --- Mediterranean Water Buffalo --- in vitro embryo production --- laparoscopic ovum pickup --- accelerated genetic gain --- prepubertal --- embryo development --- wisent --- European bison --- oocytes --- sperm --- embryos --- embryo transfer --- germplasm bank --- threatened species

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Rabbit livestock is especially important in the Mediterranean area and in the semi-arid zones of Africa, Asia, and America, representing a valuable alternative source of good animal protein of high quality (low fat and low cholesterol). However, rabbit husbandry should adapt to be a competitive and sustainable sector. In addition, it must maintain a balance between rabbit welfare, customer demands, and breeder requirements. Then, improvement in breeding systems is needed and may involve avoiding hormones and antibiotics in rabbit management; enhancing the efficiency of reproductive procedures such as artificial insemination systems and freezing of semen and embryos; extending the productive lifespan of does; improving genetic selection systems; and adjusting nutritional systems to optimize the physiological status of animals and the immune response. This Special Issue concerns all these challenges in increasing rabbit productivity and sustainability.

Research & information: general --- Biology, life sciences --- Animals & society --- Oryctolagus cuniculus --- SNPs --- SLAF-seq --- genome-wide association study --- growth trait --- myeloperoxidase --- lysozyme --- rabbits --- viral infection --- rabbit hemorrhagic disease --- goji berries --- rabbit --- insulin resistance --- leptin --- non-esterified fatty acids --- pregnancy --- lactation --- body condition score --- principal component analysis --- gene expression --- endometrium --- oviduct --- spermatozoa --- seminal plasma --- inflammation --- angiogenesis --- corpus luteum --- reproduction --- genomic selection --- imputation --- litter size --- genomic simulation --- artificial insemination --- antibiotics --- antibacterial activity --- semen quality --- reproductive performance --- sperm quality --- cryopreservation --- animal model --- assisted reproductive technology --- CTSS --- granulosa cells --- proliferation --- apoptosis --- hormone secretion --- body composition --- fertility --- kits born alive --- rabbit does --- feed intake --- free tri-iodothyronine --- thyroxine --- insulin --- glucose --- corticosterone --- NEFA --- fetus --- placenta